NCM´s restart the healing process and accelerate the formation of Granulation tissue and Epithelization1,2
Cell adhesion to microspheres in the wound bed results in morphological and functional cell changes that restart healing1,3,4

NCM’s provide a multi-point contact surface for cellular attachment.3

The Negative Charge in NCM’s surface improves cellular attachment and interaction.5



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Chronic wound situation without NCM’s:
Chronic wounds instead of progressing through the four phases of healing become ‘stuck’ in a prolonged inflammatory phase.

Healing wound treated with NCM’s:
Application of NCM's appears to trigger a synchronized series of events that shift the wound from the inflammatory to the proliferative phase, stimulating patient's own cells to restart the wound healing process.1, 3

NCM (Negatively Charged Microspheres, 5µm)
(e.g IL-1 MMPs)
Pro-inflammatory cytokines
(e.g IL-1, TNF)
Epithelial cells
Endothelial cells
Growth factors
(e.g. PDGF, VEGF)
NCM´s interact with the main cell lines involved in the healing process: macrophages, fibroblasts, endothelial cells and keratinocytes.1, 6
The cellular attachment to NCM’s results in its activation, proliferation and migration.1, 3, 6
This stimulates collagen synthesis and angiogenesis, restoring the extracellular matrix.1, 2, 3, 6
In addition, NCM´s adsorb onto their surface the excess of MMP’s, reducing the extracellular matrix degradation.3, 7
The formation of healthy granulation tissue along with neoangiogenesis leads to reduction of wound size.1, 2, 3, 6
At the end, epithelization, contraction and stable scar tissue is formed.1, 2


Acute wounds normally heal in an orderly manner by progressing through four overlapping phases: hemostasis, inflammation, proliferation and remodeling.

Throughout these phases, components of the extracellular matrix play an important role in regulating and integrating many key processes of healing.


Please, see below the main publications about the mechanism of action of PolyHeal® Micro.

1. Correa LG, et al. Negatively charged microspheres provide an additional surface for cell attachment leading to proliferation, tissue regeneration and wound healing (Poster). Presented in European Wound Management Association Congress (EWMA) 2017.

Link to publication

1. Shoham Y, et al. Wound 'dechronification' with negatively-charged polystyrene microspheres: a double-blind RCT. J Wound Care. 2013 Mar; 22(3):144-55.

2. Weissman O, et al. Post-facelift flap necrosis treatment using charged polystyrene microspheres. Can J Plast Surg. 2013; 21(1):45-7.

3. Govrin J, et al. New method for treating hard-to-heal wounds: clinical experience with charged polystyrene microspheres. Wounds UK. 2010; 6(4); 52-61.

4. Carré A, et al. How Substrate Properties Control Cell Adhesion. A Physical–Chemical Approach, Journal of Adhesion Science and Technology. 2010; 24:5, 815-830.

5. Khatua D, et al. Influence of Charge Densities of Randomly Sulfonated Polystyrene Surfaces on Cell Attachment and Proliferation. Journal of Nanoscience and Nanotechnology. 2011; Vol. 11, 4227–4230.

6. Weissman O, et al. Treatment of wounds following breast reduction and mastopexy with subsequent wound dehiscence with charged polystyrene microspheres. Wounds. 2014; 26(2):37-42.

7. Renò F, et al. Adsorption of matrix metalloproteinases onto biomedical polymers: a new aspect in biological acceptance. J Biomater Sci Polym Ed. 2008; 19(1):19-29.

8. Guest JF, et al. Cost-effectiveness of using Polyheal compared with surgery in the management of chronic wounds with exposed bones and/or tendons due to trauma in France, Germany and the UK. Int Wound J. 2015 Feb; 12(1):70-82.